Lab Testing and References

Lab testing

We have performed extensive lab tests for a wide range of parameters including those listed below. For further information and techniques used please contact us.

Biosurfactants

  • Antibacterial (including S. aureus, E. coli, C. acnes)
  • Anti-fungal (C. albicans, M. furfur)
  • Antioxidant efficacy
  • Moisturising and anti-inflammatory properties
  • Anti-mite (demodex)
  • Biofilm disruption
  • Surface tension reduction
  • Emulsifying properties
  • Foaming properties

Fermented lipids

  • Enhancement of antioxidant properties
  • Flavonoid content
  • Skin absorption
  • Stability and texture benefits
  • Lipid profile

Research articles

BIOSURFACTANTS

  1. Bueno‐Mancebo, J., Barrena, R., Artola, A., Gea, T. and Deisi Altmajer‐Vaz (2024). Surfactin as an ingredient in cosmetic industry: Benefits and trends. International Journal of Cosmetic Science. doi:https://doi.org/10.1111/ics.12957. Google.com. (2014). US20160030322A1
  2. Applications of Surfactin in Cosmetic Products – Google Patents. [online] Available at: https://patents.google.com/patent/US20160030322A1/en [Accessed 2 Feb. 2025]. Ahmadi-Ashtiani, H.-R., Baldisserotto, A., Cesa, E., Manfredini, S., Sedghi Zadeh, H., Ghafori Gorab, M., Khanahmadi, M., Zakizadeh, S., Buso, P. and Vertuani, S. (2020).
  3. Microbial Biosurfactants as Key Multifunctional Ingredients for Sustainable Cosmetics. Cosmetics, [online] 7(2), p.46. doi:https://doi.org/10.3390/cosmetics7020046. Bueno‐Mancebo, J., Barrena, R., Artola, A., Gea, T. and Deisi Altmajer‐Vaz (2024).
  4. Surfactin as an ingredient in cosmetic industry: Benefits and trends. International Journal of Cosmetic Science. doi:https://doi.org/10.1111/ics.12957. Ruan, Q., Wang, M.-P., Zou, Y., Lin, C., Cai, D. and Wang, J.-M. (2020).
  5. Interfacial stabilization of aqueous foam based on soy protein-rhamnolipids interacting mixture. 153, pp.112587–112587. doi:https://doi.org/10.1016/j.indcrop.2020.112587. Lourith, N. and Kanlayavattanakul, M. (2009).
  6. Natural surfactants used in cosmetics: glycolipids. International Journal of Cosmetic Science, 31(4), pp.255–261. doi:https://doi.org/10.1111/j.1468-2494.2009.00493.x. Ndlovu, T., Rautenbach, M., Vosloo, J.A., Khan, S. and Khan, W. (2017).
  7. Characterisation and antimicrobial activity of biosurfactant extracts produced by Bacillus amyloliquefaciens and Pseudomonas aeruginosa isolated Lawrence, J. (2014).
  8. Surfactants and Emulsifiers – dermascope. [online] dermascope. Available at: https://www.dermascope.com/surfactants-and-emulsifiers/ [Accessed 2 Feb. 2025]. Cho, W.Y., Ng, J.F., Yap, W.H. and Goh, B.H. (2022).

  9. Sophorolipids—Bio-Based Antimicrobial Formulating Agents for Applications in Food and Health. Molecules, [online] 27(17), p.5556. doi:https://doi.org/10.3390/molecules27175556.

  10. Google.com. (1995). EP0820273B1 – Use of sophorolipids in cosmetic and dermatological compositions – Google Patents. [online] Available at: https://patents.google.com/patent/EP0820273B1/en [Accessed 2 Feb. 2025]. Filipe, G.A., Bigotto, B.G., Baldo, C., Gonçalves, M.C., Kobayashi, R.K.T., Lonni, A.A.S.G. and Celligoi, M.A.P.C. (2022).

  11. Development of a multifunctional and self-preserving cosmetic formulation using sophorolipids and palmarosa essential oil against acne-causing bacteria. Journal of Applied Microbiology, [online] 133(3), pp.1534–1542. doi:https://doi.org/10.1111/jam.15659. Roberto, M., Magri, A., Baldo, C., Doumit Camilios-Neto and Antonia, M. (2015).

  12. Review: Sophorolipids A Promising Biosurfactant and it’s Applications. [online] 6(2), pp.161–174. Available at: https://www.researchgate.net/publication/276270446_Review_Sophorolipids_A_Promising_Biosurfactant_and_it. Google.com. (2024). Sign in – Google Accounts. [online] Available at: https://patents.google.com/patent/US6057302A/en [Accessed 2 Feb. 2025]. Adu, S.A., Twigg, M.S., Naughton, P., Marchant, R. and Banat, I.M. (2023).

  13. Glycolipid Biosurfactants in Skincare Applications: Challenges and Recommendations for Future Exploitation. 28(11), pp.4463–4463. doi:https://doi.org/10.3390/molecules28114463. Pal, S., Chatterjee, N., Das, A.K., McClements, D.J. and Dhar, P. (2023). Sophorolipids: A comprehensive review on properties and applications. Advances in Colloid and Interface Science, 313, p.102856. doi:https://doi.org/10.1016/j.cis.2023.102856. Abdel-Mawgoud, A.M., Lépine, F. and Déziel, E. (2010).

  14. Rhamnolipids: diversity of structures, microbial origins and roles. Applied Microbiology and Biotechnology, 86(5), pp.1323–1336. doi:https://doi.org/10.1007/s00253-010-2498-2. Google.com. (2000).

  15. US7262171B1 – Use of rhamnolipids in wound healing, treating burn shock, atherosclerosis, organ transplants, depression, schizophrenia and cosmetics – Google Patents. [online] Available at: https://patents.google.com/patent/US7262171B1/en [Accessed 2 Feb. 2025]. Develter, D.W.G. and Lauryssen, L.M.L. (2010).

  16. Properties and industrial applications of sophorolipids. European Journal of Lipid Science and Technology, 112(6), pp.628–638. doi:https://doi.org/10.1002/ejlt.200900153. Xu, R., Ma, L., Chen, T. and Wang, J. (2022).

  17. Sophorolipid Suppresses LPS-Induced Inflammation in RAW264.7 Cells through the NF-κB Signaling Pathway. Molecules, 27(15), p.5037. doi:https://doi.org/10.3390/molecules27155037. Adu, S.A., Naughton, P.J., Marchant, R. and Banat, I.M. (2020).

  18. Microbial Biosurfactants in Cosmetic and Personal Skincare Pharmaceutical Formulations. Pharmaceutics, 12(11), p.1099. doi:https://doi.org/10.3390/pharmaceutics12111099.

 

FERMENTED LIPIDS

  1. Ciardiello, T., Pinto, D., Marotta, L., Giuliani, G. and Rinaldi, F. (2020). Effects of Fermented Oils on Alpha-Biodiversity and Relative Abundance of Cheek Resident Skin Microbiota. Cosmetics, 7(2), p.34. doi:https://doi.org/10.3390/cosmetics7020034.
  2. Yu Ri Woo and Hei Sung Kim (2024). Interaction between the microbiota and the skin barrier in aging skin: a comprehensive review. Frontiers in Physiology, 15. doi:https://doi.org/10.3389/fphys.2024.1322205
  3. “Clinical Studies on Fermented Skincare: Unveiling the Benefits.” 2024. Theyeastwecando.com. December 2024. https://theyeastwecando.com/studies-on-fermented-skincare/?utm_source=chatgpt.com.
  4. Lee, D.E., Huh, C.S., and Ra, J., 2018. Cosmeceutical importance of fermented plant extracts: A short review. Journal of Applied Pharmaceutical Science, 8(07), pp.124–131.‌
  5. Pérez-Rivero, C. and José Pablo López‐Gómez (2023). Unlocking the Potential of Fermentation in Cosmetics: A Review. Fermentation, 9(5), pp.463–463. doi:https://doi.org/10.3390/fermentation9050463.
  6. Tan, Y., Gao, M., Li, L., Jiang, H., Liu, Y., Gu, T. and Zhang, J. (2024). Functional components and antioxidant activity were improved in ginger fermented by Bifidobacterium adolescentis and Monascus purpureus. Lebensmittel-Wissenschaft + Technologie/Food science & technology, pp.115931–115931. doi:https://doi.org/10.1016/j.lwt.2024.115931.

    Gharby, S., Oubannin, S., Ait Bouzid, H., Bijla, L., Ibourki, M., Gagour, J., Koubachi, J., Sakar, E.H., Majourhat, K., Lee, L.-H., Harhar, H. and Bouyahya, A. (2022).

  7. An Overview on the Use of Extracts from Medicinal and Aromatic Plants to Improve Nutritional Value and Oxidative Stability of Vegetable Oils. Foods, 11(20), p.3258. doi:https://doi.org/10.3390/foods11203258.

     

GELLAN GUM

  1. Busto, F., Licini, C., Luccarini, A., Damiani, E., Mattioli-Belmonte, M., Cometa, S. and De Giglio, E. (2023). Oleuropein-Rich Gellan Gum/Alginate Films as Innovative Treatments against Photo-Induced Skin Aging. Molecules (Basel, Switzerland), [online] 28(11), p.4352. doi:https://doi.org/10.3390/molecules28114352.
  2. Sousa, P., Moreira, A., Lopes, B., Sousa, A.C., Coelho, A., Rêma, A., Balça, M., Atayde, L., Mendonça, C., da Silva, L.P., Costa, C., Marques, A.P., Amorim, I., Alvites, R., Batista, F., Mata, F., Transmontano, J. and Maurício, A.C. (2025). Honey, Gellan Gum, and Hyaluronic Acid as Therapeutic Approaches for Skin Regeneration. Biomedicines, [online] 13(2), p.508. doi:https://doi.org/10.3390/biomedicines13020508
  3. Google.com. (2011). US20120149783A1 – Water-in-oil emulsion compositions containing gellan gum for topical delivery of active ingredients to the skin or mucosa – Google Patents. [online] Available at: https://patents.google.com/patent/US20120149783 [Accessed 11 Apr. 2025].
  4. Google.com. (1993). US5747022A – Cosmetic mask – Google Patents. [online] Available at: https://patents.google.com/patent/US5747022A/en [Accessed 11 Apr. 2025]
  5. Mahdi, M.H., Conway, B.R. and Smith, A.M. (2015). Development of mucoadhesive sprayable gellan gum fluid gels. International Journal of Pharmaceutics, 488(1-2), pp.12–19. doi:https://doi.org/10.1016/j.ijpharm.2015.04.011.‌
  6. Ter Horst, B., Moakes, R.J.A., Chouhan, G., Williams, R.L., Moiemen, N.S. and Grover, L.M. (2019). A gellan-based fluid gel carrier to enhance topical spray delivery. Acta biomaterialia, [online] 89, pp.166–179. doi:https://doi.org/10.1016/j.actbio.2019.03.036.

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